Anti-GAPDH Rabbit Polyclonal Antibody 抗GAPDH多克隆抗体
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产品编号：BE0024 品牌 : Easybio/柏奥易杰 CAS号： |
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<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">产品属性
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| 来源宿主 | Rabbit |
| 试验方法 | WB IHC IF |
| 标记 | 无 |
| 类别 | 一抗 |
Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDHare useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Product Name:GAPDH Rabbit Polyclonal antibody
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Immunogen: Full-lengthGAPDH protein of human.
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Purification:antigen affinity purification
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Tested applications: WB IHC
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Recommended dilutions: WB: 1:2000-5000 IHC: 1:200
Optimal dilutions/concentrations should be determined by the end user.
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Molecular Weight: 37KD
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Form<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">:Liquid
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% <strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">sodium azide and 50% glycerol
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Storage: GAPDH PAB is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
<strong "="" style="margin: 0px; padding: 0px; list-style: none; font-size: 12px;">Reactivity:H, R, M, Mk, Dg, Ch Hm Rabbit, Zebrafish, Xenopus
Western blot analysis of Hela cell lysate(lane1) , Rat brain tissue lysate(lane2) and Mouse brain tissue lysate(lane3) with GAPDH PAB (BE0024) diluted at 1:5000.
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